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In Vitro Cells

Repeatable in-vitro mammalian cell culture requires pure and  high quality culture media, clean and healthy cell lines, a precise and well maintained CO2 incubator, and good general aseptic culture technique.  Major culture categories include:

  • General small volume research
  • Small volume research on structural cells requiring hypoxic growth conditions
  • Medium-volume research on adherent cells
  • High volume research and scale-up on adherent cells
  • High volume research and scale-up on suspended cells

Only general government and best-practice guidelines have been applied to this type of research.  The one exception is human In Vitro fertilization, which is regulated by government bodies such as the US FDA.  Generally, incubators marketed for this purpose within the United States must be registered with the FDA as a Class II device.

To assure consistent and meaningful results, incubators used for these types of research need to feature tight parameter control, uniformity and stability over long periods of time.   Depending on type of cell being cultured, parameters may include elevated CO2, suppressed O2, elevated temperature, and elevated humidity.  Common issues to avoid include chamber contamination and loss of temperature control.  The former is typically caused by contaminated cell lines or media, poor transfer technique, or insufficient incubator cleaning/decontamination/sterilization.  The latter is a particular issue in non-refrigerated chambers being used with heat-generating cell agitation equipment (rollers, rockers, shakers, stirrers, etc) .

Other types of cell culture, including bacteriological and non-mammalian in-vitro, are often performed in similar chambers.  CO2 is usually not required, but refrigeration may be for lower temperature ectothermic organisms.  Humidification is usually not required for bacteria, given their short culture cycles.

(Marietta, OH, September 13, 2017) Caron is pleased to introduce the new 7540 Series Photostability chambers, now the fastest way to attain critical ICH Q1B (option 2) exposure levels.  Full ICH visible and UVA exposures are achievable in as little as 42 hours, less than half the time of many competing chambers.  Caron’s ground-up dedicated Q1B chamber also achieves high uniformity, providing over 75% usable shelf space.  Test more samples in less time, accelerating drug development and reducing testing costs.  

 

Separate fluorescent-powered UVA and visible light banks provide maximum intensity, while minimizing potential parameter overshoot and thermal stress. Caron even offers an integral 5°C cycle, for testing of refrigerated drugs, vaccines, and biologicals.

 

These new chambers are easy to use, simple to validate, and straightforward to service.  Equipped with an intuitive yet powerful color touchscreen display, the 7540 Series provides “manual-free” operation, programming, and adjustment.  Slide-out component modules make replacing bulbs and other parts simple, and keep the units’ benchtop footprint small.